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ab 2295862 il1β 8516 r d systems mab 201  (R&D Systems)


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    Structured Review

    R&D Systems ab 2295862 il1β 8516 r d systems mab 201
    Ab 2295862 Il1β 8516 R D Systems Mab 201, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+il1%CE%B2/Human+IL-1+beta%2FIL-1F2+Antibody/pmc11734740__sciadv__adn2811_sm-191-47-50
    Average 95 stars, based on 89 article reviews
    ab 2295862 il1β 8516 r d systems mab 201 - by Bioz Stars, 2026-09
    95/100 stars

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    Comparison of the influence of a-PVA-SPION on whole blood cytokine secretion by immune cells of RA patients and healthy donors. Notes: Supernatants were collected from whole blood survival assays (blood samples obtained from patients with RA [n=9, left] or healthy donors [n=4, right]). One example for <t>IL1β</t> secretion is given in detail in ( A ), and a tabular overview for all measured cytokines is presented in ( B ). Red arrow: cytokines that were significantly increased at SPION concentration 100 μg/mL compared to untreated control; black arrow: cytokines significantly increased at any other SPION concentration than 100 μg/mL compared to untreated control; green arrow: cytokines that remained unchanged; nd: not detectable; data are given as box and whiskers; whiskers represent minimum to maximum; one-way ANOVA: *** P <0.001; ** P <0.01; * P <0.05. Abbreviations: RA, rheumatoid arthritis; ANOVA, analysis of variance; Ctrl, control; LPS, lipopolysaccharide; PHA, Phaseolus vulgaris ; HD, healthy donors; a-PVA-SPION, amino-polyvinyl alcohol coated superparamagnetic iron oxide nanoparticles.
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    Comparison of the influence of a-PVA-SPION on whole blood cytokine secretion by immune cells of RA patients and healthy donors. Notes: Supernatants were collected from whole blood survival assays (blood samples obtained from patients with RA [n=9, left] or healthy donors [n=4, right]). One example for IL1β secretion is given in detail in ( A ), and a tabular overview for all measured cytokines is presented in ( B ). Red arrow: cytokines that were significantly increased at SPION concentration 100 μg/mL compared to untreated control; black arrow: cytokines significantly increased at any other SPION concentration than 100 μg/mL compared to untreated control; green arrow: cytokines that remained unchanged; nd: not detectable; data are given as box and whiskers; whiskers represent minimum to maximum; one-way ANOVA: *** P <0.001; ** P <0.01; * P <0.05. Abbreviations: RA, rheumatoid arthritis; ANOVA, analysis of variance; Ctrl, control; LPS, lipopolysaccharide; PHA, Phaseolus vulgaris ; HD, healthy donors; a-PVA-SPION, amino-polyvinyl alcohol coated superparamagnetic iron oxide nanoparticles.

    Journal: International Journal of Nanomedicine

    Article Title: Effects of PVA coated nanoparticles on human immune cells

    doi: 10.2147/IJN.S75936

    Figure Lengend Snippet: Comparison of the influence of a-PVA-SPION on whole blood cytokine secretion by immune cells of RA patients and healthy donors. Notes: Supernatants were collected from whole blood survival assays (blood samples obtained from patients with RA [n=9, left] or healthy donors [n=4, right]). One example for IL1β secretion is given in detail in ( A ), and a tabular overview for all measured cytokines is presented in ( B ). Red arrow: cytokines that were significantly increased at SPION concentration 100 μg/mL compared to untreated control; black arrow: cytokines significantly increased at any other SPION concentration than 100 μg/mL compared to untreated control; green arrow: cytokines that remained unchanged; nd: not detectable; data are given as box and whiskers; whiskers represent minimum to maximum; one-way ANOVA: *** P <0.001; ** P <0.01; * P <0.05. Abbreviations: RA, rheumatoid arthritis; ANOVA, analysis of variance; Ctrl, control; LPS, lipopolysaccharide; PHA, Phaseolus vulgaris ; HD, healthy donors; a-PVA-SPION, amino-polyvinyl alcohol coated superparamagnetic iron oxide nanoparticles.

    Article Snippet: Anti-human-IL1β-PE, anti-human-CD3-Pacific-blue, anti-human-CD14-APC-Cy7, anti-human-CD19-PE-Cy7, and Annexin V/7AAD apoptosis kit were from BD Biosciences (San Jose, CA, USA) and anti-human-CD15-APC was from Miltenyi Biotech GmbH (Bergisch Gladbach, Germany).

    Techniques: Concentration Assay

    Impact of a-PVA-SPION on the number and distribution of IL1β positive cells. Notes: Whole blood samples obtained from healthy donors (n=6) were incubated for 20 hours at varying a-PVA-SPION concentrations. Cellular transport was blocked by adding Brefeldin A and cells were stained for extracellular markers as well as for intracellular IL1β. Normalization according to cell count was performed and data were visualized with the help of a heat map. Data are given as number of IL1β positive cells. Abbreviations: Ctrl, control; LPS, lipopolysaccharide; PHA, Phaseolus vulgaris ; a-PVA-SPION, amino-polyvinyl alcohol coated superparamagnetic iron oxide nanoparticles.

    Journal: International Journal of Nanomedicine

    Article Title: Effects of PVA coated nanoparticles on human immune cells

    doi: 10.2147/IJN.S75936

    Figure Lengend Snippet: Impact of a-PVA-SPION on the number and distribution of IL1β positive cells. Notes: Whole blood samples obtained from healthy donors (n=6) were incubated for 20 hours at varying a-PVA-SPION concentrations. Cellular transport was blocked by adding Brefeldin A and cells were stained for extracellular markers as well as for intracellular IL1β. Normalization according to cell count was performed and data were visualized with the help of a heat map. Data are given as number of IL1β positive cells. Abbreviations: Ctrl, control; LPS, lipopolysaccharide; PHA, Phaseolus vulgaris ; a-PVA-SPION, amino-polyvinyl alcohol coated superparamagnetic iron oxide nanoparticles.

    Article Snippet: Anti-human-IL1β-PE, anti-human-CD3-Pacific-blue, anti-human-CD14-APC-Cy7, anti-human-CD19-PE-Cy7, and Annexin V/7AAD apoptosis kit were from BD Biosciences (San Jose, CA, USA) and anti-human-CD15-APC was from Miltenyi Biotech GmbH (Bergisch Gladbach, Germany).

    Techniques: Incubation, Staining, Cell Counting

    Influence of a-PVA-SPION on the concentration of secreted cytokines in whole blood samples obtained from RA patients

    Journal: International Journal of Nanomedicine

    Article Title: Effects of PVA coated nanoparticles on human immune cells

    doi: 10.2147/IJN.S75936

    Figure Lengend Snippet: Influence of a-PVA-SPION on the concentration of secreted cytokines in whole blood samples obtained from RA patients

    Article Snippet: Anti-human-IL1β-PE, anti-human-CD3-Pacific-blue, anti-human-CD14-APC-Cy7, anti-human-CD19-PE-Cy7, and Annexin V/7AAD apoptosis kit were from BD Biosciences (San Jose, CA, USA) and anti-human-CD15-APC was from Miltenyi Biotech GmbH (Bergisch Gladbach, Germany).

    Techniques: Concentration Assay

    Influence of a-PVA-SPION on the concentration of secreted cytokines in whole blood samples obtained from HD

    Journal: International Journal of Nanomedicine

    Article Title: Effects of PVA coated nanoparticles on human immune cells

    doi: 10.2147/IJN.S75936

    Figure Lengend Snippet: Influence of a-PVA-SPION on the concentration of secreted cytokines in whole blood samples obtained from HD

    Article Snippet: Anti-human-IL1β-PE, anti-human-CD3-Pacific-blue, anti-human-CD14-APC-Cy7, anti-human-CD19-PE-Cy7, and Annexin V/7AAD apoptosis kit were from BD Biosciences (San Jose, CA, USA) and anti-human-CD15-APC was from Miltenyi Biotech GmbH (Bergisch Gladbach, Germany).

    Techniques: Concentration Assay